isolation and identification of local bacillus isolates for xylanase biosynthesis.
نویسندگان
چکیده
background and objectives: bacillus species are attractive industrial organisms due to their rapid growth rates leading to a short fermentation cycle and for their capacity to secrete important enzymes and proteins such as xylanase into the extracellular medium. considering the industrial importance of xylanase, in this current study, bacillus spp. were isolated from different soils and were screened for their xylanase production. materials and methods: bacillus isolates used in this study were obtained from a national screening program carried out during 2006-2007 in which soil samples that covered areas throughout the interior of syria were collected. the prepared inoculum from each of bacillus isolates was aliquoted onto xylan agar plates, incubated at 30°c for 72 h and screened for xylanase synthesis. results: xylanolytic isolates were selected depending on the clear zones of xylan hydrolysis. fifteen isolates having the highest clearing zone were determined and grown in a solid state fermentation. of the 15 isolates, three bacilli namely sy30a, sy185c and sy190e that showed maximum xylanase production, were identified using the 16s rdna sequencing method. according to 16s rdna gene sequence data, the closest phylogenetic neighbor for sy30a was bacillus pumilus and for sy185c and sy190e isolates was bacillus subtilis . optimal ph and temperature for xylanase activity was 7.0 and 55°c for sy30a and 6.0 and 60°c for sy185c and sy190e, respectively. under these conditions, the following activities were found to be around 1157 ± 58, 916 ± 46 and 794 ± 39 (u/g) for sy30a, sy185c and sy190e, respectivly. conclusions: selected local bacillus isolates were found to be a potential source of xylanase which was proven to be quite suitable for multiple biotechnological applications. these isolates might after extensive optimization steps be an alternative to commercially available strains.
منابع مشابه
Isolation and identification of local Bacillus isolates for xylanase biosynthesis
BACKGROUND AND OBJECTIVES Bacillus species are attractive industrial organisms due to their rapid growth rates leading to a short fermentation cycle and for their capacity to secrete important enzymes and proteins such as xylanase into the extracellular medium. Considering the industrial importance of xylanase, in this current study, Bacillus spp. were isolated from different soils and were scr...
متن کاملIsolation and identification of Bacillus cereus of dry bulk and packaged vegetables by culture and PCR
Background: Bacillus cereus is an endemic, Gram-positive, rod-shaped, motile, beta hemolytic bacterium. Some strains are harmful to humans and cause foodborne illness and it's spore is resistant against difficult condition In recent years life style and feeding habits have changed and an increase in the use of ready to eat product has raised concern about quality and health assessment. Dehydrat...
متن کاملIsolation and identification of xylanase producing fungal isolate
Fungal isolate SATU 1 producing xylanase was isolated from decaying wood and soil sample collected from Tumkur region. The fungal isolate was identified by microscopic and other conventional methods. The selected potent fungal isolate SATU 1 was screened on Czepox dox agar containing (1% xylan) as a substrate. Isolate SATU 1 showed maximum zone of 46 mm on screening media. SATU1 showed maximum ...
متن کاملIdentification of novel indigenous Bacillus thuringiensis isolates.
Internally Transcribed Spacers (ITS) characterization and distribution of Repetitive Extragenic Palindromic (REP) sequences were studied in the genome of 223 field isolates of Bacillus thuringiensis from Madurai, India. They were characterized by morphological, biochemical and molecular methods. One hundred and twenty four of a total 223 isolates fitted ITS characterization of B. thuringiensis ...
متن کاملIsolation, identification, and antimicrobial susceptibility of Clostridium perfringens isolates from acute necrotic enteritis of broiler chickens
The aim of this study was to isolate, identify and determine the antimicrobial susceptibility of Clostridium perfringens (CP) isolates from acute necrotic enteritis of broiler chickens. All broiler carcasses diagnosed as necrotic enteritis (NE) were sampled, subjected to microbial tests and 40 isolates were identified according to standard procedures. The antimicrobial susceptibility of CP isol...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of microbiologyجلد ۶، شماره ۲، صفحات ۱۲۷-۱۳۲
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023